- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-49629r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Ubiquitin carboxyl-terminal hydrolase BAP1,Bap1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Ubiquitin carboxyl- terminal hydrolase BAP1, BAP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
BAP1,HUCEP-13; UCHL2; hucep-6; cerebral protein 6; cerebral protein-13; BRCA1 associated protein-1
Search name
Rat BAP1 ELISA KIT ,Rat HUCEP-13 ELISA KIT ,Rat UCHL2 ELISA KIT ,Rat hucep-6 ELISA KIT ,Rat cerebral protein 6 ELISA KIT ,Rat cerebral protein-13 ELISA KIT ,Rat BRCA1 associated protein-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Ubiquitin carboxyl- terminal hydrolase BAP1, BAP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin carboxyl-terminal hydrolase BAP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase BAP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase BAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin carboxyl-terminal hydrolase BAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验A Method for Assaying Deubiquitinating Enzymes
). The purified Ub-specific protease-6 (yUBP-6) in yeast and cUBP41, Ub C-terminal hydrolase-1 (cUCH-1), cUCH-6, and cUCH-8 in chick skeletal muscle were prepared as described (13 , 15 -18 ). Ub-PESTc was purified from an E. coli strain AR13 carrying pNMHUB-PESTc
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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