Rat Cluster of differentiation

Rat Cluster of differentiation 40 ligand, CD40L ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
CD40 ligand(CD40-L),Tumor necrosis factor ligand superfamily member 5,T-cell antigen Gp39,TNF-related activation protein(TRAP), CD40
ligand,CD154; CD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; gp39; hCD40L; CD40 antigen ligand; CD40-L; T-B cell-activating molecule;

Search name
Rat CD40 ligand ELISA Kit, Rat CD40-L ELISA Kit, Rat Tumor necrosis factor ligand superfamily member 5 ELISA Kit, Rat Cluster of
differentiation 40 ligand ELISA Kit, Rat CD40L ELISA Kit, Rat T-cell antigen Gp39 ELISA Kit, Rat TNF-related activation protein ELISA Kit,
Rat TRAP ELISA Kit, Rat CD154 ELISA Kit, Rat HIGM1 ELISA Kit, Rat IGM ELISA Kit, Rat IMD3 ELISA Kit, Rat T-BAM ELISA Kit, Rat TNFSF5
ELISA Kit, Rat gp39 ELISA Kit, Rat hCD40L ELISA Kit, Rat CD40 antigen ligand ELISA Kit, Rat T-B cell-activating molecule ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat cd40 ligand,CD40-L concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD40-L. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD40-L and Avidin conjugated to Horseradish
Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that
contain CD40-L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.
The concentration of CD40-L in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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