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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Pyruvate dehydrogenase E1 component subunit alpha,testis-s
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Pyruvate dehydrogenase E1 component subunit alpha, testis- specific form, mitochondrial, PDHA2 ELISA KIT
96 Tests
Operating instruction
Nova lifetech®
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial,; PDHE1-A type II,PDHA2,PDHAL; PDHE1-A type II; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific; pyruvate dehydrogenase (lipoamide) alpha 2
Search name
Rat Pyruvate dehydrogenase E1 component subunit alpha ELISA KIT ,Rat testis-specific form ELISA KIT ,Rat mitochondrial ELISA KIT ,Rat ELISA KIT ,Rat PDHE1-A type II ELISA KIT ,Rat PDHA2 ELISA KIT ,Rat PDHAL ELISA KIT ,Rat PDHE1-A type II ELISA KIT ,Rat pyruvate dehydrogenase E1 component subunit alpha ELISA KIT ,Rat testis-specific form ELISA KIT ,Rat mitochondrial ELISA KIT ,Rat pyruvate dehydrogenase ELISA KIT ,Rat E1-alpha polypeptide ELISA KIT ,Rat testis specific ELISA KIT ,Rat pyruvate dehydrogenase (lipoamide) alpha 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial,Pdha2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pdha2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pdha2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pdha2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pdha2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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