Rat Serine/threonine- protein kinase Nek7, NEK7 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NEK7,serine/threonine-protein kinase Nek7; NIMA (never in mitosis gene a)-related kinase 7; never in mitosis A-related kinase 7; nimA-related protein kinase 7; NIMA-related kinase 7
Search name
Rat NEK7 ELISA KIT ,Rat serine/threonine-protein kinase Nek7 ELISA KIT ,Rat NIMA (never in mitosis gene a)-related kinase 7 ELISA KIT ,Rat never in mitosis A-related kinase 7 ELISA KIT ,Rat nimA-related protein kinase 7 ELISA KIT ,Rat NIMA-related kinase 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Serine/threonine- protein kinase Nek7, NEK7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEK7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NEK7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NEK7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NEK7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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