- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-38752r
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2,Srd5a2 ELISA K
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat 3- oxo- 5- alpha- steroid 4- dehydrogenase 2, Srd5a2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Srd5a2,5 alpha-SR2; S5AR 2; SR type 2; steroid 5-alpha-reductase 2; type II 5-alpha reductase; steroid-5-alpha-reductase alpha polypeptide 2 ,3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2,Steroid 5-alpha-reductase 2,S5AR 2,5 alpha-SR2,SR type 2,
Search name
Rat Srd5a2 ELISA KIT ,Rat 5 alpha-SR2 ELISA KIT ,Rat S5AR 2 ELISA KIT ,Rat SR type 2 ELISA KIT ,Rat steroid 5-alpha-reductase 2 ELISA KIT ,Rat type II 5-alpha reductase ELISA KIT ,Rat steroid-5-alpha-reductase alpha polypeptide 2 ELISA KIT ,Rat 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2 ELISA KIT ,Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 ELISA KIT ,Rat Steroid 5-alpha-reductase 2 ELISA KIT ,Rat S5AR 2 ELISA KIT ,Rat 5 alpha-SR2 ELISA KIT ,Rat SR type 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2,3-oxo-5-alpha-steroid 4-dehydrogenase 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-oxo-5-alpha-steroid 4-dehydrogenase 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 3-oxo-5-alpha-steroid 4-dehydrogenase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 3-oxo-5-alpha-steroid 4-dehydrogenase 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-oxo-5-alpha-steroid 4-dehydrogenase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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