相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Polyribonucleotide 5'-hydroxyl-kinase Clp1,Clp1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HEAB,pre-mRNA cleavage complex II protein Clp1; polyribonucleotide 5-hydroxyl-kinase Clp1; polynucleotide kinase Clp1; polyadenylation factor Clp1; CLP1, cleavage and polyadenylation factor I subunit, homolog; hClp1; ATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; cleavage and polyadenylation factor I subunit 1
Search name
Rat HEAB ELISA KIT ,Rat pre-mRNA cleavage complex II protein Clp1 ELISA KIT ,Rat polyribonucleotide 5-hydroxyl-kinase Clp1 ELISA KIT ,Rat polynucleotide kinase Clp1 ELISA KIT ,Rat polyadenylation factor Clp1 ELISA KIT ,Rat CLP1 ELISA KIT ,Rat cleavage and polyadenylation factor I subunit homolog ELISA KIT ,Rat hClp1 ELISA KIT ,Rat ATP/GTP-binding protein ELISA KIT ,Rat homolog of yeast CFIA subunit Clp1p ELISA KIT ,Rat cleavage and polyadenylation factor I subunit 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CLP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CLP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒? dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好,我是新手,你用过的ELISA试剂在哪买的,哪个公司的比较好啊?还有,测定大鼠脑组织里的cAMP,放免法和ELISA法各有什么优缺点啊?谢谢回复!! songdianwei 我在 R&D公司买的,测
组织充分那就可以用这些试剂盒检测。 当然,不同公司cAMP试剂盒灵敏度有些差异,这个也需要做好选择。 另外,选择这些试剂盒时还需要根据自己实验室的仪器配置来选择,例如Cisbio,跟PE公司的cAMP试剂盒是基于TR-FRET方法的,因此酶标仪必须有TRF跟FRET功能;Promega公司的试剂盒是化学发光的,因此仪器上需要有Luminescence; DiscoverX的试剂盒也是测的Luminescence;其他一些公司就是Elisa试剂盒,这个大家就比较
h,同上收集培养上清液 用双抗体夹心ELISA 方法测HSC-T6培养上清液中PⅢP ,CⅣ的含量。以上每组均设3个复孔,具体操作严格按照试剂盒说明进行。 1.3 统计学方法 采用SPSS12.0统计软件,进行Spearman相关分析,单因素ANOVA(方差分析),两样本比较采用LSD-t检验,实验数据均以(x±s)表示,P<0.05判定有显著性差异。 2 结果 2.1 HMGB1对 HSC-T6分泌PⅢP、CⅣ的剂量效应 HMGB1能促进
技术资料暂无技术资料 索取技术资料






