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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat PAXIP1-associated protein 1,Pa1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat PAXIP1- associated protein 1, PA1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PA1,PAGR1,C16orf53; GAS; PAXIP1-associated protein 1; PTIP-associated 1 protein; PTIP-associated protein 1; glutamate-rich coactivator associated with SRC1; glutamate-rich coactivator interacting with SRC1/NCOA1; PAXIP1 associated glutamate-rich protein 1
Search name
Rat PA1 ELISA KIT ,Rat PAGR1 ELISA KIT ,Rat C16orf53 ELISA KIT ,Rat GAS ELISA KIT ,Rat PAXIP1-associated protein 1 ELISA KIT ,Rat PTIP-associated 1 protein ELISA KIT ,Rat PTIP-associated protein 1 ELISA KIT ,Rat glutamate-rich coactivator associated with SRC1 ELISA KIT ,Rat glutamate-rich coactivator interacting with SRC1/NCOA1 ELISA KIT ,Rat PAXIP1 associated glutamate-rich protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of ratPAXIP1-associated protein 1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toPAXIP1-associated protein 1, During the reaction,PAXIP1-associated protein 1in the sample or standard competes with a fixed amount of biotin-labeledPAXIP1-associated protein 1 for sites on a pre-coated Monoclonal antibody specific to PAXIP1-associated protein 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAXIP1-associated protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
protein levels should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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