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文献和实验Dynamics of Claudins Expression in Colitis and Colitis-Associated Cancer in Rat
Claudins comprise a multigene family of 24 species and have been shown to constitute the backbone of tight junction strands in simple epithelial cells and to be directly involved in their barrier functions. Apical-most tight junction protein
Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
A PDE2A2-associated protein kinase phosphorylates PDE2A2 in vivo and in vitro to inhibit its catalytic activity. Rat brain PDE2A2 may be solubilized using nona (ethylene glycol) mono dodecyl ether (Lubrol 12A9). PDE2A2 exists in a complex
Cell death detection in Xenopus embryos by ELISA
(eppendorf) for 10 min. at 4°C Remove 160 µl supernatant / embryo, avoiding pellet contamination of the supernatant Samples can be stored, if not used the same day, in aliquots at -20°C Do ELISA according to manufacturer's instructions.
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