- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0890r
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Pulmonary surfatcant-associated protein A
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Pulmonary surfatcant- associated protein A ELISA Kit
Product Name:Rat Pulmonary surfatcant- associated protein A ELISA Kit
Packing:96T
Catalog No.:ELA-E0890r
Gene Name:Rat SP- A
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rat Pulmonary surfatcant- associated protein A
Alternative Name:Rat SP- A ELISA KIT, Rat Pulmonary surfatcant- associated protein A ELISA KIT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Pulmonary surfatcant- associated protein A ELISA Kit allows for the in vitro quantitative determination ofRat Pulmonary surfatcant- associated protein A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat Pulmonary surfatcant- associated protein A ELISA Kit has been pre-coated with an Rat SP- A antibody specific to Rat Pulmonary surfatcant- associated protein A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Pulmonary surfatcant- associated protein A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Pulmonary surfatcant- associated protein A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Pulmonary surfatcant- associated protein A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Cigarette Smoke Exposure as a Model of Inflammation Associated with COPD
of a rat model of chronic obstructive pulmonary disease (COPD) induced by sidestream cigarette smoke. Toxicol. Lett. 189:225‐234. GO TO THE FULL PROTOCOL: PDF or HTML
upon a known amino acid sequence of the target protein, virulence-associated protein A (VapA) of the bacterium Rhodococcus equi , an important pulmonary pathogen in foals. The peptides were biotinylated and used in an ELISA to screen immune sera
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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