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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Orexin receptor type 2,Hcrtr2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Orexin receptor type 2, HCRTR2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Ox2R,hypocretin receptor type 2; orexin type-2 receptor; ox-2-R; ox2-R; hypocretin receptor 2, Orexin receptor type 2, orexin receptor 2
Search name
Rat Ox2R ELISA KIT ,Rat hypocretin receptor type 2 ELISA KIT ,Rat orexin type-2 receptor ELISA KIT ,Rat ox-2-R ELISA KIT ,Rat ox2-R ELISA KIT ,Rat hypocretin receptor 2 ELISA KIT ,Rat Orexin receptor type 2 ELISA KIT ,Rat orexin receptor 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofratOx2Rconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ox2R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forOx2R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ox2R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ox2R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验secondary antibody review -- data from 99 publications
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