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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Serine protease inhibitor Kazal-type 3,Spink3
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Serine protease inhibitor Kazal- type 3, Spink3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Spink3,prostatic secretory glycoprotein; p12; serine protease inhibitor Kazal-type 3; serine protease inhibitor, Kazal type 3; serine peptidase inhibitor, Kazal type 3
Search name
Rat Spink3 ELISA KIT ,Rat prostatic secretory glycoprotein ELISA KIT ,Rat p12 ELISA KIT ,Rat serine protease inhibitor Kazal-type 3 ELISA KIT ,Rat serine protease inhibitor Kazal type 3 ELISA KIT ,Rat serine peptidase inhibitor ELISA KIT ,Rat Kazal type 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Serine protease inhibitor Kazal- type 3, Spink3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spink3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Spink3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Spink3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Spink3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验) or early death. The serine protease uPA, in concert with its inhibitor PAI-1, promotes tumor cell adhesion, migration, and proliferation, as well as extracellular matrix degradation and, thus, facilitates tumor cell invasion and metastasis. The various
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
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