- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-30269r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Regulator of G-protein signaling 7,Rgs7
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Regulator of G- protein signaling 7, RGS7 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat RGS7 ELISA KIT ,Rat regulator of G-protein signaling RGS7 ELISA KIT ,Rat regulator of G-protein signalling 7 ELISA KIT ,Rat regulator of G-protein signaling 7 ELISA KIT
Intended use
Rat RGS7 ELISA kit allows for the in vitro quantitative determination of Rat RGS7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat RGS7 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RGS7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RGS7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RGS7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat RGS7 ELISA KIT manual request by email:
Order RGS7 ELISA KIT by email:
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文献和实验secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
【申请上传】Current Protocols in Immunology
in Microtiter Plates for Determination of [3H]Thymidine Uptake 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures 3F Obtaining Human Peripheral Blood Cells 3G Cryopreservation of Cells 3H Dialysis and Concentration of Protein Solutions 3I
含量成反比。 本试剂盒用于检测在GPCR激动剂刺激下活细胞或者细胞膜制备品产生的cAMP。对于偶联Gαs的受体，激动剂刺激导致665nm的荧光强度降低，而拮抗剂则可以逆转这一效应；对于偶联Gαi的受体，在激动剂刺激的同时用forskolin刺激cAMP产生，那么激动剂则抑制forskolin诱导的cAMP的生成，因此对照只给forskolin的细胞组可以通过665nm荧光强度的增加反应激动剂的效应。 该试剂盒的灵敏度很高，室温下反应在20h内是稳定的。本试剂盒
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