- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-08447r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Mast cell protease 2,Mcpt2
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Mast cell protease 2, Mcpt2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Mcpt2,rMCP-2,Mast cell protease 2,Group-specific protease;Mast cell protease II,rMCP-II, MCT2
Search name
Rat Mcpt2 ELISA KIT ,Rat rMCP-2 ELISA KIT ,Rat Mast cell protease 2 ELISA KIT ,Rat Group-specific protease ELISA KIT ,Rat Mast cell protease II ELISA KIT ,Rat rMCP-II ELISA KIT ,Rat MCT2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat rMCP-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to rMCP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for rMCP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain rMCP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of rMCP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Endothelial Cell Activation by Mast Cell Mediators
products synergize endothelial cell inflammatory responses (see Note 1 ). The described methods outline: (1) the collection of rat peritoneal mast cells; (2) preparation of MCGs; (3) co-culture of human endothelial cells with mast cell granules
【申请上传】Current Protocols in Immunology
Degranulation of Mast Cells Unit 7.27 51Cr Release Assay of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC Unit 7.28 Measurement of Cellular Adhesion Under Static Conditions Unit 7.29 Cell Adhesion Under Hydrodynamic Flow Conditions Unit 7.30 Isolation
that degrades laminin and type IV collagen and stimulates protease-activated receptor-2. J. Neurochem. (2000), 74(4), 1731�1738, Sawada, K. et al. 2. Purification of mast cell proteases from murine skin. Exp. Dermatol. (1999), 8(5), 413�418
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