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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat C-X-C chemokine receptor type 4,Cxcr4
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat C-X-C chemokine receptor type 4, CXCR4 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
C-X-C chemokine receptor type 4,CXC-R4,CXCR-4,NPYRL,Leukocyte-derived seven transmembrane domain receptor,LESTR,LCR1,HM89,Stromal cell-derived factor 1 receptor,SDF-1 receptor,FB22,Fusin,CXCR4,CXC-R4,CD184; D2S201E; FB22; HM89; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; C-X-C chemokine receptor type 4; CD184 antigen; SDF-1 receptor; fusin; leukocyte-derived seven transmembrane domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; stromal cell-derived factor 1 receptor; chemokine C-X-C motif receptor 4
Search name
Rat C-X-C chemokine receptor type 4 ELISA KIT ,Rat CXC-R4 ELISA KIT ,Rat CXCR-4 ELISA KIT ,Rat NPYRL ELISA KIT ,Rat Leukocyte-derived seven transmembrane domain receptor ELISA KIT ,Rat LESTR ELISA KIT ,Rat LCR1 ELISA KIT ,Rat HM89 ELISA KIT ,Rat Stromal cell-derived factor 1 receptor ELISA KIT ,Rat SDF-1 receptor ELISA KIT ,Rat FB22 ELISA KIT ,Rat Fusin ELISA KIT ,Rat CXCR4 ELISA KIT ,Rat CXC-R4 ELISA KIT ,Rat CD184 ELISA KIT ,Rat D2S201E ELISA KIT ,Rat FB22 ELISA KIT ,Rat HM89 ELISA KIT ,Rat HSY3RR ELISA KIT ,Rat LAP3 ELISA KIT ,Rat LCR1 ELISA KIT ,Rat LESTR ELISA KIT ,Rat NPY3R ELISA KIT ,Rat NPYR ELISA KIT ,Rat NPYRL ELISA KIT ,Rat NPYY3R ELISA KIT ,Rat WHIM ELISA KIT ,Rat C-X-C chemokine receptor type 4 ELISA KIT ,Rat CD184 antigen ELISA KIT ,Rat SDF-1 receptor ELISA KIT ,Rat fusin ELISA KIT ,Rat leukocyte-derived seven transmembrane domain receptor ELISA KIT ,Rat lipopolysaccharide-associated protein 3 ELISA KIT ,Rat neuropeptide Y receptor Y3 ELISA KIT ,Rat seven transmembrane helix receptor ELISA KIT ,Rat seven-transmembrane-segment receptor spleen ELISA KIT ,Rat stromal cell-derived factor 1 receptor ELISA KIT ,Rat chemokine C-X-C motif receptor 4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat c-x-c chemokine receptor type 4,CXC-R4 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXC-R4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CXC-R4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CXC-R4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CXC-R4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
Khawaja, X., Evans, N., Reilly, Y., Ennis, C., and Minchin, M.C. 1995. Characterization of the binding of [3H]WAY‐100635, a novel 5‐hydroxytryptamine‐1A receptor antagonist, to rat brain. J. Neurochem. 64:2716‐2726
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
Characterization of Chemokine Receptors
., DiGregorio, D., Mak, J.Y., Horuk, R., and Schall, T.J. 1993. Molecular cloning, functional expression, and signaling characteristics of a C‐C chemokine receptor. Cell. 72:415‐425.
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