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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat C-X-C chemokine receptor type 2,Cxcr2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat C- X- C chemokine receptor type 2, CXCR2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CXCR2,CXC-R2; CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB; C-X-C chemokine receptor type 2; CXCR-2; CXCR2 gene for IL8 receptor type B; GRO/MGSA receptor; IL-8 receptor type 2; IL-8R B; chemokine (CXC) receptor 2; high affinity interleukin-8 receptor B; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin 8 receptor beta; interleukin-8 receptor type B; chemokine (C-X-C motif) receptor 2 C-X-C chemokine receptor type 2,CXC-R2;CXCR-2,High affinity interleukin-8 receptor B,IL-8R B,
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat C-X-C chemokine receptor type 2, CXC-R2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXC-R2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CXC-R2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CXC-R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CXC-R2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
Khawaja, X., Evans, N., Reilly, Y., Ennis, C., and Minchin, M.C. 1995. Characterization of the binding of [3H]WAY‐100635, a novel 5‐hydroxytryptamine‐1A receptor antagonist, to rat brain. J. Neurochem. 64:2716‐2726
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
Characterization of Chemokine Receptors
., DiGregorio, D., Mak, J.Y., Horuk, R., and Schall, T.J. 1993. Molecular cloning, functional expression, and signaling characteristics of a C‐C chemokine receptor. Cell. 72:415‐425.
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