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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Membrane metallo endopeptidase,MME
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Membrane metallo endopeptidase, MME ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CD10,neprilysin; CALLA; NEP; SFE; atriopeptidase; common acute lymphocytic leukemia antigen; enkephalinase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; neutral endopeptidase 24.11; skin fibroblast elastase; membrane metallo-endopeptidase,Neprilysin,Skin fibroblast elastase,SFE,Neutral endopeptidase 24.11,NEP,Neutral endopeptidase,Enkephalinase,Atriopeptidase,Common acute lymphocytic leukemia antigen,CALLA,
Search name
Rat CD10 ELISA KIT ,Rat neprilysin ELISA KIT ,Rat CALLA ELISA KIT ,Rat NEP ELISA KIT ,Rat SFE ELISA KIT ,Rat atriopeptidase ELISA KIT ,Rat common acute lymphocytic leukemia antigen ELISA KIT ,Rat enkephalinase ELISA KIT ,Rat membrane metallo endopeptidase ELISA KIT ,Rat neutral endopeptidase enkephalinase ELISA KIT ,Rat membrane metallo-endopeptidase ELISA KIT ,Rat neutral endopeptidase enkephalinase ELISA KIT ,Rat CALLA ELISA KIT ,Rat CD10 ELISA KIT ,Rat membrane metallo-endopeptidase variant 1 ELISA KIT ,Rat membrane metallo-endopeptidase variant 2 ELISA KIT ,Rat neutral endopeptidase 24.11 ELISA KIT ,Rat skin fibroblast elastase ELISA KIT ,Rat membrane metallo-endopeptidase ELISA KIT ,Rat Neprilysin ELISA KIT ,Rat Skin fibroblast elastase ELISA KIT ,Rat SFE ELISA KIT ,Rat Neutral endopeptidase 24.11 ELISA KIT ,Rat NEP ELISA KIT ,Rat Neutral endopeptidase ELISA KIT ,Rat Enkephalinase ELISA KIT ,Rat Atriopeptidase ELISA KIT ,Rat Common acute lymphocytic leukemia antigen ELISA KIT ,Rat CALLA ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Neprilysin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neprilysin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neprilysin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neprilysin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
MSP119S, 500 mg/L, α-MF, effective468Plasmodium falciparum subtilisin-like protease pfsub1I, 0.5g/L449Plasmodium vivax apical membrane antigen I (AMA-1)S, 50 mg/L, PHO1[94]Ryschosporium secalis avirulence gene product NIP1I, 16 mg/L, PHO1, active334
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