- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-05497r
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Suppressor of cytokine signaling 3,Socs3
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Suppressor of cytokine signaling 3, Socs3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SOCS3,ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3; suppressor of cytokine signaling 3, SOCS-3
Search name
Rat SOCS3 ELISA KIT ,Rat ATOD4 ELISA KIT ,Rat CIS3 ELISA KIT ,Rat Cish3 ELISA KIT ,Rat SOCS-3 ELISA KIT ,Rat SSI-3 ELISA KIT ,Rat SSI3 ELISA KIT ,Rat STAT-induced STAT inhibitor 3 ELISA KIT ,Rat cytokine-inducible SH2 protein 3 ELISA KIT ,Rat suppressor of cytokine signaling 3 ELISA KIT ,Rat SOCS-3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat suppressor of cytokine signaling 3,SOCS-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOCS-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SOCS-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SOCS-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOCS-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验【申请上传】Current Protocols in Immunology
Immunocompromised Animals Unit 1.3 Handling and Restraint Unit 1.4 Anesthesia Unit 1.5 Animal Identification Section II Use of Mouse, Rat, Hamster, and Rabbit Unit 1.6 Parenteral Injections Unit 1.7 Blood Collection Unit 1.8 Euthanasia Section III Selected
对肝纤维化的发生、发展发挥重要作用呢？我们课题组的先前研究表明在肝纤维大鼠的肝组织中HMGB1的表达明显增强，但HMGB1是否通过激活HSC参与肝纤维化的发生发展目前尚不清楚，本研究拟通过HMGB1刺激HSC以观察ECM的合成情况来进一步探讨HMGB1与肝纤维化的关系。 1 材料与方法 1.1 主要试剂 HMGB1由本室制备保存；新生胎牛血清（FCS）为杭州四季青生物工程材料研究所产品；高糖型DMEM购自英国Gibco公司；大鼠PⅢP ，CⅣ双抗体夹心ELISA检测试剂盒购自
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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