Rat Neural cell adhesion molec

Rat Neural cell adhesion molecule 1, NCAM1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Neural cell adhesion molecule 1,N-CAM-1,NCAM-1, CD56,neural cell adhesion molecule 1; MSK39; NCAM; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule, NCAM, MSK39;

Search name

Rat Neural cell adhesion molecule 1 ELISA KIT ,Rat N-CAM-1 ELISA KIT ,Rat NCAM-1 ELISA KIT ,Rat CD56 ELISA KIT ,Rat neural cell adhesion molecule 1 ELISA KIT ,Rat MSK39 ELISA KIT ,Rat NCAM ELISA KIT ,Rat antigen recognized by monoclonal antibody 5.1H11 ELISA KIT ,Rat neural cell adhesion molecule ELISA KIT ,Rat NCAM ELISA KIT ,Rat MSK39 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat neural cell adhesion molecule 1,N-CAM-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-CAM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for N-CAM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-CAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N-CAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.