- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-03992r
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Neural cell adhesion molecule L1,L1cam
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Neural cell adhesion molecule L1, L1CAM ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Neural cell adhesion molecule L1,N-CAM-L1,NCAM-L1, L1CAM,NCAM-L1; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; S10; SPG1; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1; L1 cell adhesion molecule
Search name
Rat Neural cell adhesion molecule L1 ELISA KIT ,Rat N-CAM-L1 ELISA KIT ,Rat NCAM-L1 ELISA KIT ,Rat L1CAM ELISA KIT ,Rat NCAM-L1 ELISA KIT ,Rat CAML1 ELISA KIT ,Rat CD171 ELISA KIT ,Rat HSAS ELISA KIT ,Rat HSAS1 ELISA KIT ,Rat MASA ELISA KIT ,Rat MIC5 ELISA KIT ,Rat N-CAM-L1 ELISA KIT ,Rat N-CAML1 ELISA KIT ,Rat S10 ELISA KIT ,Rat SPG1 ELISA KIT ,Rat antigen identified by monoclonal antibody R1 ELISA KIT ,Rat neural cell adhesion molecule L1 ELISA KIT ,Rat L1 cell adhesion molecule ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat neural cell adhesion molecule l1,N-CAM-L1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-CAM-L1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for N-CAM-L1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-CAM-L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N-CAM-L1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验神经细胞粘着分子(neural cell adhesion molecule,N-CAM)
N-CAM是膜糖蛋白,至少以三种形式存在,但都是由同一基因编码。其中两种是跨膜蛋白,第三种共价结合在细胞质膜的外表面。无论是何种存在方式,N-CAM分子都有一部分伸出到细胞外表面。三种N-CAM在细胞外的结构都一样,都含有与细胞粘着相关的结合位点。 将分离纯化的N-CAM加入到人工磷脂脂质体中, 这种人工脂质体能够相互粘着,但是加入了相应的抗体就不会发生粘着, 不加N-CAM的人工脂质体也不会发生粘着。由此可以推测:细胞粘着是由细胞质膜中的蛋白分子介导的,N
细胞粘着分子(cell adhesion molecule, CAM)
参与细胞粘着的分子称为细胞粘着分子。 自从N-CAM被发现之后,陆续发现了一些新的细胞粘着分子,如Ng-CAM是神经胶质细胞粘着分子,L-CAM是肝细胞的细胞粘着分子,I-CAM是普遍存在的一种细胞粘着分子,它的配体是LFA-1;而LEC-CAM是白细胞及其它循环细胞中发现的细胞粘着分子。上述细胞粘着分子都是糖蛋白,在细胞外结构域都有与肽共价结合的糖基。根据细胞粘着分子的作用方式可分为三个家族:①免疫球蛋白超家族,如V-CAM、Ng-CAM、I-CAM和L1等;②钙粘着蛋白家族，如E
Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition
CD2 is a cell adhesion molecule that mediates T-cell activation by binding to its ligand CD58 on antigen-presenting cells. Interaction between CD2 and CD58 or leukocyte function-associated antigen-3 (LFA-3) helps to optimize immune
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