- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-03549r
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Prolyl 4-hydroxylase subunit alpha-1,P4ha1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Prolyl 4- hydroxylase subunit alpha- 1, P4HA1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
P4HA1,P4HA; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline, 2-oxoglutarate 4-dioxygenase ,proline 4-hydroxylase alpha polypeptide I; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; prolyl 4-hydroxylase alpha polypeptide I; prolyl 4-hydroxylase subunit alpha 1, Prolyl 4-hydroxylase subunit alpha-1,4-PH alpha-1
Search name
Rat P4HA1 ELISA KIT ,Rat P4HA ELISA KIT ,Rat C-P4Halpha(I) ELISA KIT ,Rat collagen prolyl 4-hydroxylase alpha(I) ELISA KIT ,Rat procollagen-proline ELISA KIT ,Rat 2-oxoglutarate 4-dioxygenase ELISA KIT ,Rat proline 4-hydroxylase alpha polypeptide I ELISA KIT ,Rat procollagen-proline ELISA KIT ,Rat 2-oxoglutarate-4-dioxygenase subunit alpha-1 ELISA KIT ,Rat prolyl 4-hydroxylase alpha polypeptide I ELISA KIT ,Rat prolyl 4-hydroxylase subunit alpha 1 ELISA KIT ,Rat Prolyl 4-hydroxylase subunit alpha-1 ELISA KIT ,Rat 4-PH alpha-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat prolyl 4-hydroxylase subunit alpha-1,4-PH alpha-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-PH alpha-1, During the reaction, 4-PH alpha-1 in the sample or standard competes with a fixed amount of biotin-labeled 4-PH alpha-1 for sites on a pre-coated Monoclonal antibody specific to 4-PH alpha-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-PH alpha-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
mg/L, authentic526, 527Fibroblast collagenase (proMMP-1)S, 2.3 mg/L, α-MF[229]Type I collagenI, 0.5 g/L, active528, 540Type III collagen (with prolyl 4-hydroxylase)I, 15 mg/L[288], 540C1r complement serine proteinaseS, 1.5 mg/L, ,α-MF, active434C1
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