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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Carbonic anhydrase 2,Ca2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat carbonic anhydrase 2, CA2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Carbonic anhydrase 2,; Carbonate dehydratase II,Carbonic anhydrase II(CA-II),; Car2; AI131712; CAII; Ca2; Car-2; Ltw-5; Lvtw-5; CA II;
CA-II;
Search name
Rat carbonic anhydrase 2 ELISA Kit, Rat CA-2 ELISA Kit, Rat Carbonate dehydratase II ELISA Kit, Rat CA-II
ELISA Kit, Rat Car2 ELISA Kit, Rat AI131712 ELISA Kit, Rat Car-2 ELISA Kit, Rat Ltw-5 ELISA Kit, Rat
CA2 ELISA Kit, Rat Lvtw-5 ELISA Kit, Rat CA II ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Carbonic anhydrase 2 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic anhydrase 2. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carbonic
anhydrase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Carbonic anhydrase 2, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carbonic
anhydrase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒? dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好,我是新手,你用过的ELISA试剂在哪买的,哪个公司的比较好啊?还有,测定大鼠脑组织里的cAMP,放免法和ELISA法各有什么优缺点啊?谢谢回复!! songdianwei 我在 R&D公司买的,测
组织充分那就可以用这些试剂盒检测。 当然,不同公司cAMP试剂盒灵敏度有些差异,这个也需要做好选择。 另外,选择这些试剂盒时还需要根据自己实验室的仪器配置来选择,例如Cisbio,跟PE公司的cAMP试剂盒是基于TR-FRET方法的,因此酶标仪必须有TRF跟FRET功能;Promega公司的试剂盒是化学发光的,因此仪器上需要有Luminescence; DiscoverX的试剂盒也是测的Luminescence;其他一些公司就是Elisa试剂盒,这个大家就比较
不同,应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子:比如检测一抗为单抗的mouse anti rat CD11b,clone OX-42 purified IgG,那么它的isotype 是mouse IgG2a, 所以可以用purified(纯化的) mouse IgG2a来做OX-42的同型对照(Isotype Control)。一般的的生物技术公司和国内的代理都有出售。 同型对照:是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类,若使用直接
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