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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat neutrophil gelatinase-associated lipocalin/ lipocalin 2,LC
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat neutrophil gelatinase- associated lipocalin/ lipocalin 2, LCN2/ NGAL ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Rat LCN2 ELISA KIT ,Rat siderocalin LCN2 ELISA KIT ,Rat 24p3 ELISA KIT ,Rat MSFI ELISA KIT ,Rat NGAL ELISA KIT ,Rat p25 ELISA KIT ,Rat 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA KIT ,Rat lipocalin-2 ELISA KIT ,Rat migration-stimulating factor inhibitor ELISA KIT ,Rat oncogene 24p3 ELISA KIT ,Rat lipocalin 2 ELISA KIT ,Rat Neutrophil gelatinase-associated lipocalin ELISA KIT
Intended use
Rat LCN2 ELISA KIT allows for the in vitro quantitative determination of Rat neutrophil gelatinase- associated lipocalin/ lipocalin 2, LCN2/ NGAL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this Rat LCN2 ELISA KIT has been pre-coated with an antibody specific to LCN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LCN2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LCN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 15.6-1000pg/ml
Rat LCN2 ELISA KIT manual download
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文献和实验人中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin, NGAL)是Kjeldsen等人于1993年在中性粒细胞的特殊颗粒中首先发现的。以往的研究表明NGAL与炎症、胚胎发育、免疫应答、趋化作用、信号转导以及多种肿瘤的发生与发展等过程密切相关。NGAL在乳腺癌、食管癌、结直肠癌、胃癌、慢性粒细胞性白血病等细胞系中均过表达,并在癌细胞的浸润与不良分化中发挥重要的作用。进一步研究证实,NGAL是存在于中性粒细胞和某些上皮
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
mg/L, active502Feline type I interferonsS, α-MF, active518Goat α-lactalbuminS, α-MF, 20 mg/L506Hen lysozymeS, 20 mg/L, α-MF[159]Herring antifreeze proteinS, α-MF, active377Mammalian amyloid precursor fusion proteinS, α-MF, active378Mammalian lipocalin
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