- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E1258r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat glutamic acid decarboxylase,GAD
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat glutamic acid decarboxylase, GAD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Glutamate decarboxylase 1,67 kDa glutamic acid decarboxylase(GAD-67),Glutamate decarboxylase 67 kDa isoform, GAD1, CPSQ1;
GAD; SCP; GAD-67; glutamate decarboxylase 1;
Search name
Rat Glutamate decarboxylase 1 ELISA Kit, Rat glutamic acid decarboxylase ELISA Kit, Rat GAD ELISA Kit, Rat GAD-67 ELISA Kit, Rat Glutamate
decarboxylase 67 kDa isoform ELISA Kit, Rat GAD1 ELISA Kit, Rat CPSQ1 ELISA Kit, Rat SCP ELISA Kit, Rat glutamate decarboxylase 1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat glutamate decarboxylase 1,Glutamate decarboxylase 1
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate decarboxylase 1. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Glutamate decarboxylase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Glutamate decarboxylase 1, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Glutamate decarboxylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验人群。如 IDDM 的亲属和一般人群中具有遗传素质者，此时免疫学标志尚未出现，可用遗传基因标志检测。二期即免疫破坏期，此时血清内已有抗胰岛细胞的自身抗体出现，是 IDDM 预防的关键。三期即 IDDM 发病的早期，已出现糖尿病症状，但胰岛β细胞尚有一定功能，干预治疗可保护残存的胰岛β细胞不被破坏或延缓破坏。 IDDM 检测指标较多，主要是胞浆胰岛细胞抗体（ islet cell autoantibodies,IAAS ）和谷氨脱羧酶抗体（ glutamic acid decarboxylase antibody
This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). A. PROTOCOL 1. prepare platelets
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
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