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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat glutamic acid decarboxylase autoantibody
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Glutamic Acid Decarboxylase Autoantibody, GAD-Ab ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutamic Acid Decarboxylase Autoantibody,GAD-Ab, Anti-GAD,anti Glutamic Acid Decarboxylase,Anti GAD,anti Glutamate decarboxylase,
Search name
Rat Glutamic Acid Decarboxylase Autoantibody ELISA KIT ,Rat GAD-Ab ELISA KIT ,Rat Anti-GAD ELISA KIT ,Rat anti Glutamic Acid Decarboxylase ELISA KIT ,Rat Anti GAD ELISA KIT ,Rat anti Glutamate decarboxylase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Glutamic Acid Decarboxylase Autoantibody,GAD-Ab concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antigen specific to GAD-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen preparation specific for GAD-Ab and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GAD-Ab, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GAD-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). A. PROTOCOL 1. prepare platelets
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
antiplatelet autoantibodies from autoimmune thrombocytopenic purpura-prone (NZW x BXSB)F1 mice. Blood 82(3): 837-44. ELISA相关仪器试剂设备 酶标仪 洗板机 化学发光检测仪 ELISA检测试剂盒
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