Rat Thrombin activatable fibri

Rat Thrombin activatable fibrinolysis inhibitor, TAFI ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Carboxypeptidase B2,Thrombin-activable fibrinolysis inhibitor,TAFI,Carboxypeptidase U,CPU,Plasma carboxypeptidase B,pCPB, CPB2,TAFI; CPU; PCPB; carboxypeptidase B-like protein; carboxypeptidase B2; carboxypeptidase B2,plasma carboxypeptidase U;carboxypeptidase R; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase B2

Search name

Rat Carboxypeptidase B2 ELISA KIT ,Rat Thrombin-activable fibrinolysis inhibitor ELISA KIT ,Rat TAFI ELISA KIT ,Rat Carboxypeptidase U ELISA KIT ,Rat CPU ELISA KIT ,Rat Plasma carboxypeptidase B ELISA KIT ,Rat pCPB ELISA KIT ,Rat CPB2 ELISA KIT ,Rat TAFI ELISA KIT ,Rat CPU ELISA KIT ,Rat PCPB ELISA KIT ,Rat carboxypeptidase B-like protein ELISA KIT ,Rat carboxypeptidase B2 ELISA KIT ,Rat carboxypeptidase B2 ELISA KIT ,Rat plasma carboxypeptidase U;carboxypeptidase R ELISA KIT ,Rat thrombin-activable fibrinolysis inhibitor ELISA KIT ,Rat thrombin-activatable fibrinolysis inhibitor ELISA KIT ,Rat carboxypeptidase B2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Carboxypeptidase B2 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase B2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carboxypeptidase B2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carboxypeptidase B2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carboxypeptidase B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.