Rat Aspartate aminotransferase

Rat Aspartate aminotransferase, AST ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Aspartate aminotransferase, mitochondrial(mAspAT),Transaminase A,Plasma membrane-associated fatty acid-binding
protein(FABPpm),Fatty acid-binding protein(FABP-1),Glutamate oxaloacetate transaminase 2,GOT1,GOT,GPT,ASTQTL1; GIG18;
aspartate aminotransferase, cytoplasmic; glutamate oxaloacetate transaminase 1; growth-inhibiting protein 18; transaminase A;
glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1),mAspAT, Maat,Aspartate transaminase ,AST ,
serum glutamic oxaloacetic transaminase ,SGOT,

Search name
Rat Aspartate aminotransferase ELISA Kit, Rat mAspAT ELISA Kit, Rat Plasma membrane-associated fatty acid-binding protein
ELISA Kit, Rat FABPpm ELISA Kit, Rat Fatty acid-binding protein ELISA Kit, Rat FABP-1 ELISA Kit, Rat FABP ELISA Kit, Rat
GOT1 ELISA Kit, Rat GOT ELISA Kit, Rat Maat ELISA Kit, Aspartate aminotransferase ELISA Kit, mAspAT ELISA Kit, Plasma
membrane-associated fatty acid-binding protein ELISA Kit, FABPpm ELISA Kit, Fatty acid-binding protein ELISA Kit, FABP-1
ELISA Kit, FABP ELISA Kit, GOT1 ELISA Kit, GOT ELISA Kit, Maat ELISA Kit,Rat GOT1 ELISA Kit,GOT1 ELISA Kit,Rat ASTÂ
ELISA Kit ,Rat serum glutamic oxaloacetic transaminase ELISA Kit,Rat SGOT ELISA Kit,AST ELISA Kit ,serum glutamic
oxaloacetic transaminase ELISA Kit,SGOT ELISA Kit, Rat Aspartate aminotransferase cytoplasmic, AST ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat aspartate aminotransferase, cytoplasmic,Aspartate
aminotransferase, cytoplasmic concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological
fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate aminotransferase, cytoplasmic.
Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation
specific for Aspartate aminotransferase, cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate
well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Aspartate aminotransferase,
cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Aspartate aminotransferase, cytoplasmic in the samples is then determined by comparing the O.D. of the
samples to the standard curve.

PDF manual download