- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0257r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat procollagen III N-terminal peptide,PIIINT
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat procollagen III N- terminal peptide, PIIINT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
N-terminal propeptide of Collagen alpha-1(III) chain,PIIINP, N- terminal procollagen III propeptide
Search name
Rat PIIINP ELISA Kit, Rat N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit, Rat N- terminal procollagen III propeptide ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat N-terminal propeptide of Collagen alpha-1(III) chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-terminal propeptide of Collagen alpha-1(III) chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for N-terminal propeptide of Collagen alpha-1(III) chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-terminal propeptide of Collagen alpha-1(III) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N-terminal propeptide of Collagen alpha-1(III) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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