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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Lipoarabinomannan,LAM
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Lipoarabinomannan, LAM ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Lipoarabinomannan, LAM
Search name
Rat LAM ELISA Kit, Rat Lipoarabinomannan ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat lipoarabinomannan,Lipoarabinomannan concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Lipoarabinomannan, During the reaction, Lipoarabinomannan in the sample or standard competes
with a fixed amount of biotin-labeled Lipoarabinomannan for sites on a pre-coated Monoclonal antibody specific to Lipoarabinomannan.
Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)
is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Lipoarabinomannan in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
PDF manual download
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人 脂阿拉伯甘露聚糖(LAM) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中脂阿拉伯甘露聚糖(LAM)含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 人脂阿拉伯甘露聚糖(LAM) 水平。用纯化的 人脂阿拉伯甘露聚糖(LAM) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 脂阿拉伯甘露聚糖(LAM
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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