- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0085r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat leukemia inhibitory factor
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Leukemia inhibitory factor, LIF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LIF,Leukemia inhibitory factor,leukemia inhibitory factor; CDF; DIA; HILDA; MLPLI; D factor; cholinergic differentiation factor; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; hepatocyte-stimulating factor III; rat interleukin in DA cells; melanoma-derived LPL inhibitor
Search name
Rat LIF ELISA KIT ,Rat bLIF ELISA KIT, Rat Leukemia inhibitory factor ELISA KIT ,Rat leukemia inhibitory factor ELISA KIT ,Rat CDF ELISA KIT ,Rat DIA ELISA KIT ,Rat HILDA ELISA KIT ,Rat MLPLI ELISA KIT ,Rat D factor ELISA KIT ,Rat cholinergic differentiation factor ELISA KIT ,Rat differentiation inhibitory activity ELISA KIT ,Rat differentiation-inducing factor ELISA KIT ,Rat differentiation-stimulating factor ELISA KIT ,Rat hepatocyte-stimulating factor III ELISA KIT ,Rat rat interleukin in DA cells ELISA KIT ,Rat melanoma-derived LPL inhibitor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat leukemia inhibitory factor,LIF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LIF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro
as well as embryoid bodies when cultivated in the presence of leukemia inhibitory factor. RESC expressed stage-specific-embryonic antigen 1 and the major histocompatibility class 1 molecule. Neuronal differentiation is achieved by standard retinoic acid treatment
cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒？ dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好，我是新手，你用过的ELISA试剂在哪买的，哪个公司的比较好啊？还有，测定大鼠脑组织里的cAMP，放免法和ELISA法各有什么优缺点啊？谢谢回复！！ songdianwei 我在 R&D公司买的，测
组织充分那就可以用这些试剂盒检测。 当然，不同公司cAMP试剂盒灵敏度有些差异，这个也需要做好选择。 另外，选择这些试剂盒时还需要根据自己实验室的仪器配置来选择，例如Cisbio,跟PE公司的cAMP试剂盒是基于TR-FRET方法的，因此酶标仪必须有TRF跟FRET功能；Promega公司的试剂盒是化学发光的，因此仪器上需要有Luminescence; DiscoverX的试剂盒也是测的Luminescence；其他一些公司就是Elisa试剂盒，这个大家就比较
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