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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Glutamate receptor 2,Gria2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Glutamate receptor 2, GluR2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Gria2,GluR2, GluR-2,GLURB; AMPA 2,GluA2; GluR-K2; HBGR2; AMPA-selective glutamate receptor 2; gluR-2; gluR-B; glutamate receptor 2; glutamate receptor,ionotropic
Search name
Rat Gria2 ELISA KIT ,Rat GluR2 ELISA KIT ,Rat GluR-2 ELISA KIT ,Rat GLURB ELISA KIT ,Rat AMPA 2 ELISA KIT ,Rat GluA2 ELISA KIT ,Rat GluR-K2 ELISA KIT ,Rat HBGR2 ELISA KIT ,Rat AMPA-selective glutamate receptor 2 ELISA KIT ,Rat gluR-2 ELISA KIT ,Rat gluR-B ELISA KIT ,Rat glutamate receptor 2 ELISA KIT ,Rat glutamate receptor ELISA KIT ,Rat ionotropic ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat glutamate receptor 2,GluR-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GluR-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GluR-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GluR-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GluR-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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of the NMDA-type of glutamate receptor. More recently, Cytochrome c oxidase has emerged as a novel target for •NO, which competes with O2 for binding to this mitochondrial complex. This reaction establishes •NO not only as a regulator of cellular metabolism
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