- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- CELI-66118r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Calcitriol
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Calcitriol ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Calcitriol,1,25-dihydroxycholecalciferol,1,25-dihydroxyvitamin D3,DVD,DHVD 3
Search name
Rat Calcitriol ELISA Kit, Rat 1,25-dihydroxycholecalciferol ELISA Kit, Rat 1,25-dihydroxyvitamin D3 ELISA Kit, Rat DVD ELISA Kit, Rat DHVD 3 ELISA Kit
Intended use
Rat Calcitriol ELISA Kit allows for the in vitro quantitative determination of Rat Calcitriol concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Rat Calcitriol ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcitriol, During the reaction, Calcitriol in the sample or standard competes with a fixed amount of biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 15.6-1000pg/ml
Rat Calcitriol ELISA Kit PDF manual download
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