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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Triglyceride,TG
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Triglyceride, TG ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Triglyceride, TG,triacylglycerol,TAG,triacylglyceride
Search name
Rat Triglyceride ELISA KIT ,Rat TG ELISA KIT ,Rat triacylglycerol ELISA KIT ,Rat TAG ELISA KIT ,Rat triacylglyceride ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Triglyceride concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triglyceride, During the reaction, Triglyceride in the sample or standard competes with a fixed amount of biotin-labeled Triglyceride for sites on a pre-coated Monoclonal antibody specific to Triglyceride. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Triglyceride in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
充分摇匀后,室温放置5-10分钟,3500转/分钟,吸弃上清液,测各沉淀管的放射性计数(p)。 四.结果分析 1.参考值:TG抗体B/T≥30%为阳性。 2.临床意义:可用于自身免疫疾病的诊断。 五注意事项 1收到试剂盒后,尽快存放在2-8°C冰箱中。 2阴性对照管在数据处理时非常重要,要做复管。 3分离剂加前一定要摇匀。 4加样时应避免血清样品间的交叉污染。 5加样前试剂要平衡到室温并摇匀。 六质量控制 1每批测定都使用北京
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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