Rat Angiotensin (1-9), AGT 1-9 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Angiotensin (1-9),AGT 1-9 Angiotensin 1-9, ANHU; SERPINA8; SERPINA8; Ang I; Ang II; Ang III; Ang IV; Angiotensinogen; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; Serpin A8Cleaved into the following 8 chains:Angiotensin-1; Angiotensin 1-10; Angiotensin I; Ang IAngiotensin-2; Angiotensin 1-8; Angiotensin II; Ang IIAngiotensin-3; Alternative name(s):; Angiotensin 2-8; Angiotensin III; Ang III; Des-Asp[1]-angiotensin IIAngiotensin-4;Angiotensin 3-8; Angiotensin IV
Search name
Rat Angiotensin (1-9) ELISA KIT ,Rat AGT 1-9 Angiotensin 1-9 ELISA KIT ,Rat ANHU ELISA KIT ,Rat SERPINA8 ELISA KIT ,Rat SERPINA8 ELISA KIT ,Rat Ang I ELISA KIT ,Rat Ang II ELISA KIT ,Rat Ang III ELISA KIT ,Rat Ang IV ELISA KIT ,Rat Angiotensinogen ELISA KIT ,Rat angiotensinogen (serpin peptidase inhibitor clade A member 8 ELISA KIT ,Rat Angiotensinogen ELISA KIT ,Rat Serpin A8Cleaved into the following 8 chains:Angiotensin-1 ELISA KIT ,Rat Angiotensin 1-10 ELISA KIT ,Rat Angiotensin I ELISA KIT ,Rat Ang IAngiotensin-2 ELISA KIT ,Rat Angiotensin 1-8 ELISA KIT ,Rat Angiotensin II ELISA KIT ,Rat Ang IIAngiotensin-3 ELISA KIT ,Rat Alternative name(s): ELISA KIT ,Rat Angiotensin 2-8 ELISA KIT ,Rat Angiotensin III ELISA KIT ,Rat Ang III ELISA KIT ,Rat Des-Asp[1]-angiotensin IIAngiotensin-4;Angiotensin 3-8 ELISA KIT ,Rat Angiotensin IV ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Angiotensin (1-9) concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin (1-9), During the reaction, Angiotensin (1-9) in the sample or standard competes with a fixed amount of biotin-labeled Angiotensin (1-9) for sites on a pre-coated Monoclonal antibody specific to Angiotensin (1-9). Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Angiotensin (1-9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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