Histidyl-tRNA synthetase, cyto
plasmic, Hars ELISA KIT/ Mouse Histidyl-tRNA synthetase, cytoplasmic, Hars ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-53397m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Histidyl-tRNA synthetase, cytoplasmic, Hars
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Histidyl- tRNA synthetase, cytoplasmic, Hars ELISA KIT
Product Name:Mouse Histidyl- tRNA synthetase, cytoplasmic, Hars ELISA KIT
Packing:96T
Catalog No.:ELI-53397m
Gene Name:Mouse Hars
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Hars
Alternative Name:Mouse Histidyl-tRNA synthetase, cytoplasmic, Hars
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histidyl- tRNA synthetase, cytoplasmic, Hars ELISA KIT allows for the in vitro quantitative determination of Mouse Hars concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Histidyl- tRNA synthetase, cytoplasmic, Hars ELISA KIT has been pre-coated with an Mouse Histidyl-tRNA synthetase, cytoplasmic, Hars antibody specific to Mouse Hars .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hars and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hars , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hars in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolate It All：siRNA • miRNA • Total RNA • Native Protein
? Recover High Quality Total RNA To assess the quality of total RNA isolated with the mir Vana PARIS Kit, 1 x 106 HeLa cells were homogenized in the Cell Disruption Buffer provided with the kit. Because the homogenization is performed quickly
. No. 1 666 681), purified bovine serum albumin (BSA, Cat. No. 711 454) and E. coli tRNA (Cat. No. 109 541) were from Roche. Triton X-100 (Cat. No. T-9284), formamide (Cat. No. F-4761), sheared salmon sperm DNA (ssDNA, Cat. No. D-7656), 4',6-diamidino
Linear amplification of limiting amounts of RNA for gene expression studies
expression analysis. This approach was successfully applied to gene expression studies of the midbrain-hindbrain organizer region of the mouse but can be applied to many other systems to isolate cell populations. Using the mouse as a model organism
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