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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Small VCP
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Small VCP/p97- interacting protein, Svip ELISA KIT
Product Name:Mouse Small VCP/p97- interacting protein, Svip ELISA KIT
Packing:96T
Catalog No.:ELI-53104m
Gene Name:Mouse Svip
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Svip
Alternative Name:Mouse Small VCP/p97-interacting protein, Svip
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Small VCP/p97- interacting protein, Svip ELISA KIT allows for the in vitro quantitative determination of Mouse Svip concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Small VCP/p97- interacting protein, Svip ELISA KIT has been pre-coated with an Mouse Small VCP/p97-interacting protein, Svip antibody specific to Mouse Svip .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Svip and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Svip , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Svip in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Resources for Small Regulatory RNAs
., and Tuschl, T. 2006. A novel class of small RNAs bind to MILI protein in mouse testes. Nature 442:203‐207. Barrett, T., Troup, D.B., Wilhite, S.E., Ledoux, P., Rudnev, D
Analysis of Proteins using Small Format 2D Gel Electrophoresis
the swab against the side of the tube. The bacteria lyse within 1 or 2 minutes. Large cell debris are removed by centrifugation at 11,500 x g for 5 minutes. The supernatant is stored at -70C until required for analysis. The protein concentration
High‐Throughput RT‐PCR for Small‐Molecule Screening Assays
to measure the changes in gene expression due to perturbagens such as small molecules or siRNA. The availability of new instrumentation for liquid handling and real?time PCR analysis, as well as the commercial availability of start?to?finish kits for RT?PCR
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