- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-30868m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
H-2 class II histocompatibility antigen, E-S beta chain,
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse H- 2 class II histocompatibility antigen, E- S beta chain, H2-Eb1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
H2-Eb1,H-2Eb; H2Eb; Ia-4; Ia4; H-2 class II histocompatibility antigen, I-E beta chain; I-E beta MHC class II; MHC H2-IE-beta cell surface glycoprotein; MHC class II IE-b; cell surface glycoprotein; major histocompatibility complex H-2E beta chain; histocompatibility 2, class II antigen E beta,H-2 class II histocompatibility antigen, I-A beta chain
Search name
Mouse H2-Eb1 ELISA KIT ,Mouse H-2Eb ELISA KIT ,Mouse H2Eb ELISA KIT ,Mouse Ia-4 ELISA KIT ,Mouse Ia4 ELISA KIT ,Mouse H-2 class II histocompatibility antigen ELISA KIT ,Mouse I-E beta chain ELISA KIT ,Mouse I-E beta MHC class II ELISA KIT ,Mouse MHC H2-IE-beta cell surface glycoprotein ELISA KIT ,Mouse MHC class II IE-b ELISA KIT ,Mouse cell surface glycoprotein ELISA KIT ,Mouse major histocompatibility complex H-2E beta chain ELISA KIT ,Mouse histocompatibility 2 class II antigen E beta ELISA KIT ,Mouse H-2 class II histocompatibility antigen I-A beta chain ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse H- 2 class II histocompatibility antigen, E- S beta chain, H2-Eb1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2-Eb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for H2-Eb1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain H2-Eb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H2-Eb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA
Major histocompatibility class I and II (MHC-I and MHC-II) antigens (Ags), as well as a variety of cell surface receptors, have been shown to enter the endocytic pathway of antigen-presenting cells (APCs). MHC Ags endocytose into clathrin
to result predominately from a human anti-mouse major histocompatibility complex (MHC) class II reactivity. Our recent development of NOD-scid IL2rγ null β2m null and NOD-scid IL2rγ null Ab null stocks of mice now make it possible to investigate
Binding Kinetics of Superantigen with TCR and MHC Class II
antigen processing, they interact with the Vβ region of TCR and their binding sites lie outside the peptide-binding groove of the major histocompatibility complex (MHC) class II molecule (1 -5 ). The plasticity of TCR binding to MHC-peptide complex
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