H-2 class II histocompatibilit

Mouse H- 2 class II histocompatibility antigen, E- S beta chain, H2-Eb1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

H2-Eb1,H-2Eb; H2Eb; Ia-4; Ia4; H-2 class II histocompatibility antigen, I-E beta chain; I-E beta MHC class II; MHC H2-IE-beta cell surface glycoprotein; MHC class II IE-b; cell surface glycoprotein; major histocompatibility complex H-2E beta chain; histocompatibility 2, class II antigen E beta,H-2 class II histocompatibility antigen, I-A beta chain

Search name

Mouse H2-Eb1 ELISA KIT ,Mouse H-2Eb ELISA KIT ,Mouse H2Eb ELISA KIT ,Mouse Ia-4 ELISA KIT ,Mouse Ia4 ELISA KIT ,Mouse H-2 class II histocompatibility antigen ELISA KIT ,Mouse  I-E beta chain ELISA KIT ,Mouse I-E beta MHC class II ELISA KIT ,Mouse MHC H2-IE-beta cell surface glycoprotein ELISA KIT ,Mouse MHC class II IE-b ELISA KIT ,Mouse cell surface glycoprotein ELISA KIT ,Mouse major histocompatibility complex H-2E beta chain ELISA KIT ,Mouse histocompatibility 2 class II antigen E beta ELISA KIT ,Mouse H-2 class II histocompatibility antigen I-A beta chain ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse H- 2 class II histocompatibility antigen, E- S beta chain, H2-Eb1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2-Eb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for H2-Eb1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain H2-Eb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H2-Eb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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