Claudin-5, Cldn5 ELISA KIT/ Mo

Mouse Claudin- 5, Cldn5 ELISA KIT

Product Name:Mouse Claudin- 5, Cldn5 ELISA KIT
Packing:96T

Catalog No.:ELI-50506m

Gene Name:Mouse Cldn5

Detect Range:78.125-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Cldn5

Alternative Name:Mouse Claudin-5, Cldn5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Claudin- 5, Cldn5 ELISA KIT allows for the in vitro quantitative determination of Mouse Cldn5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Claudin- 5, Cldn5 ELISA KIT has been pre-coated with an Mouse Claudin-5, Cldn5 antibody specific to Mouse Cldn5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cldn5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cldn5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cldn5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!