- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-23864m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Carboxypeptidase D, Cpd
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Carboxypeptidase D, Cpd ELISA KIT
Product Name:Mouse Carboxypeptidase D, Cpd ELISA KIT
Packing:96T
Catalog No.:ELI-23864m
Gene Name:Mouse Cpd
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Cpd
Alternative Name:Mouse Carboxypeptidase D, Cpd
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Carboxypeptidase D, Cpd ELISA KIT allows for the in vitro quantitative determination of Mouse Cpd concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Carboxypeptidase D, Cpd ELISA KIT has been pre-coated with an Mouse Carboxypeptidase D, Cpd antibody specific to Mouse Cpd .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cpd and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cpd , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cpd in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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