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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
G protein-coupled receptor kinase 6, Grk6
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse G protein- coupled receptor kinase 6, GRK6 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GRK6,GPRK6,G protein-coupled receptor kinase GRK6,G protein-coupled receptor kinase 6
Search name
Mouse GRK6 ELISA KIT ,Mouse GPRK6 ELISA KIT ,Mouse G protein-coupled receptor kinase GRK6 ELISA KIT ,Mouse G protein-coupled receptor kinase 6 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse G protein- coupled receptor kinase 6, GRK6 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse G protein- coupled receptor kinase 6, GRK6, During the reaction, Mouse G protein- coupled receptor kinase 6, GRK6 in the sample or standard competes with a fixed amount of biotin-labeled Mouse G protein- coupled receptor kinase 6, GRK6 for sites on a pre-coated Monoclonal antibody specific to Mouse G protein- coupled receptor kinase 6, GRK6. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse G protein- coupled receptor kinase 6, GRK6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
Agonist occupation of G protein-coupled receptors (GPCRs) leads to a cellular response that wanes, or desensitizes, with prolonged agonist exposure. Different cellular mechanisms contribute to desensitization, including the altered function
Discovery and Function of the Very Large G Protein-Coupled Receptor
The very large G protein-coupled receptor 1 (VLGR1), also known as MASS1 or GPR98, is most notable among the family of adhesion GPCR for its size. Encoded by an 18.9 kb open reading frame, the ~700 kDa primary translation product is by far
Activation of MAPKs by G Protein-Coupled Receptors
G protein-coupled receptors (GPCRs) constitute the largest family of cell-surface molecules involved in signal transmission. These receptors play key physiologic roles, with their dysfunction resulting in a number of disease states
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