G protein-coupled receptor kin
ase 5, Grk5 ELISA KIT/ Mouse G protein-coupled receptor kinase 5, Grk5 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-30712m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
G protein-coupled receptor kinase 5, Grk5
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse G protein- coupled receptor kinase 5, Grk5 ELISA KIT
Product Name:Mouse G protein- coupled receptor kinase 5, Grk5 ELISA KIT
Packing:96T
Catalog No.:ELI-30712m
Gene Name:Mouse Grk5
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Grk5
Alternative Name:Mouse G protein-coupled receptor kinase 5, Grk5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse G protein- coupled receptor kinase 5, Grk5 ELISA KIT allows for the in vitro quantitative determination of Mouse Grk5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse G protein- coupled receptor kinase 5, Grk5 ELISA KIT has been pre-coated with an Mouse G protein-coupled receptor kinase 5, Grk5 antibody specific to Mouse Grk5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Grk5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Grk5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Grk5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
Agonist occupation of G protein-coupled receptors (GPCRs) leads to a cellular response that wanes, or desensitizes, with prolonged agonist exposure. Different cellular mechanisms contribute to desensitization, including the altered function
Discovery and Function of the Very Large G Protein-Coupled Receptor
The very large G protein-coupled receptor 1 (VLGR1), also known as MASS1 or GPR98, is most notable among the family of adhesion GPCR for its size. Encoded by an 18.9 kb open reading frame, the ~700 kDa primary translation product is by far
Activation of MAPKs by G Protein-Coupled Receptors
G protein-coupled receptors (GPCRs) constitute the largest family of cell-surface molecules involved in signal transmission. These receptors play key physiologic roles, with their dysfunction resulting in a number of disease states
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