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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Potassium channel subfamily U member 1, Kcnu1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Potassium channel subfamily U member 1, Kcnu1 ELISA KIT
Product Name:Mouse Potassium channel subfamily U member 1, Kcnu1 ELISA KIT
Packing:96T
Catalog No.:ELI-47893m
Gene Name:Mouse Kcnu1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Kcnu1
Alternative Name:Mouse Potassium channel subfamily U member 1, Kcnu1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Potassium channel subfamily U member 1, Kcnu1 ELISA KIT allows for the in vitro quantitative determination of Mouse Kcnu1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Potassium channel subfamily U member 1, Kcnu1 ELISA KIT has been pre-coated with an Mouse Potassium channel subfamily U member 1, Kcnu1 antibody specific to Mouse Kcnu1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kcnu1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kcnu1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kcnu1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
., Frossard, N., Kato, S., Metzger, D., Li, M., and Chambon, P. 2009. Thymic stromal lymphopoietin overproduced by keratinocytes in mouse skin aggravates experimental asthma. Proc. Natl. Acad. Sci. U.S.A. 106:1536‐1541
Quantitation Kit (#F-2962, Molecular Probes, Eugene, OR) TE 12 channel multi-pipettors Computer equipped with Microsoft Excel software Ds-DNA Standards 50 µg/ml 100 µg/ml 250 µg/ml 500 µg/ml µl TE 90 80 50 0 µl ds-DNA (0.5 mg/ml) 10 20 50 100
Expression Cloning of Neural Genes Using Xenopus laevis Oocytes
, A.M., Schuster, G., Brown, A.M., and Joho, R.H. 1989. A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature 340:642‐645.
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