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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白G-protein coupled receptor 12, Gpr12含量
- 适应物种:
小鼠
- 标记物:
G-protein coupled receptor 12, Gpr12
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse G- protein coupled receptor 12, Gpr12 ELISA KIT
Product Name:Mouse G- protein coupled receptor 12, Gpr12 ELISA KIT
Packing:96T
Catalog No.:ELI-09763m
Gene Name:Mouse Gpr12
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Gpr12
Alternative Name:Mouse G-protein coupled receptor 12, Gpr12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse G- protein coupled receptor 12, Gpr12 ELISA KIT allows for the in vitro quantitative determination of Mouse Gpr12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse G- protein coupled receptor 12, Gpr12 ELISA KIT has been pre-coated with an Mouse G-protein coupled receptor 12, Gpr12 antibody specific to Mouse Gpr12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gpr12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gpr12 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gpr12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Discovery and Function of the Very Large G Protein-Coupled Receptor
The very large G protein-coupled receptor 1 (VLGR1), also known as MASS1 or GPR98, is most notable among the family of adhesion GPCR for its size. Encoded by an 18.9 kb open reading frame, the ~700 kDa primary translation product is by far
G Protein-Coupled Receptors Disrupted in Human Genetic Disease
Genetic variation in G protein-coupled receptors (GPCRs) results in the disruption of GPCR function in a wide variety of human genetic diseases. In vitro strategies have been used to elucidate the molecular pathologies that underlie naturally
of distinct oligomeric species is monitored with a ratiometric multicolor BiFC approach. Curr. Protoc. Neurosci. 51:5.29.1?5.29.15. © 2010 by John Wiley & Sons, Inc. Keywords: G protein?coupled receptor; oligomerization; bimolecular
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