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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Microprocessor complex subunit DGCR8, Dgcr8
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Microprocessor complex subunit DGCR8, Dgcr8 ELISA KIT
Product Name:Mouse Microprocessor complex subunit DGCR8, Dgcr8 ELISA KIT
Packing:96T
Catalog No.:ELI-46781m
Gene Name:Mouse Dgcr8
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Dgcr8
Alternative Name:Mouse Microprocessor complex subunit DGCR8, Dgcr8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Microprocessor complex subunit DGCR8, Dgcr8 ELISA KIT allows for the in vitro quantitative determination of Mouse Dgcr8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Microprocessor complex subunit DGCR8, Dgcr8 ELISA KIT has been pre-coated with an Mouse Microprocessor complex subunit DGCR8, Dgcr8 antibody specific to Mouse Dgcr8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dgcr8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dgcr8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dgcr8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex
syndrome critical region 8 gene (DGCR8), which is deleted in DiGeorge syndrome (15 ,16 ). In this chapter, we detail the methods used for the biochemical isolation and identification of the Microprocessor complex from human cells. We include a protocol
Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
In animals, the Microprocessor complex cleaves primary transcripts of microRNAs (pri-miRNAs) to produce precursor microRNAs in the nucleus. The core components of Microprocessor include the Drosha ribonuclease and its RNA-binding partner
precursors.Biochem Cell Biol, 1999, 77(4): 277-91 [7] Gregory RI, Yan K, Amuthan G, et al. The microprocessor complex mediates the genesis of microRNAs. Nature, 2004,432(7014): 235-40 [8] Yeom KH, Lee Y, Han JJ, et al. Characterization of DGCR8/Pasha
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