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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Thymidylate kinase, Dtymk
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Thymidylate kinase, Dtymk ELISA KIT
Product Name:Mouse Thymidylate kinase, Dtymk ELISA KIT
Packing:96T
Catalog No.:ELI-46532m
Gene Name:Mouse Dtymk
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Dtymk
Alternative Name:Mouse Thymidylate kinase, Dtymk
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Thymidylate kinase, Dtymk ELISA KIT allows for the in vitro quantitative determination of Mouse Dtymk concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Thymidylate kinase, Dtymk ELISA KIT has been pre-coated with an Mouse Thymidylate kinase, Dtymk antibody specific to Mouse Dtymk .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dtymk and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dtymk , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dtymk in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验。 激酶活性分析 蛋白激酶通常是多个信号转导网络的常见组分,它们影响了众多负责生物反应的下游效应物,因此,评估某个特定激酶的活性可能为平行通路 提供宝贵信息。生物学样品中的激酶活性通常是在体外测定的,在ATP的存在下将免疫沉淀的激酶与外源底物共同孵育。之后通过一些报告系统来评估特定激酶对底物的磷酸化,包括显色、放射性或荧光检测。此外,R&D Systems还提供非放射性的Universal Kinase Activity Kit,能够定量任何可产生ADP的激酶的活性。尽管我们能够
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