- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-24331m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Annexin A11, Anxa11
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Annexin A11, Anxa11 ELISA KIT
Product Name:Mouse Annexin A11, Anxa11 ELISA KIT
Packing:96T
Catalog No.:ELI-24331m
Gene Name:Mouse Anxa11
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Anxa11
Alternative Name:Mouse Annexin A11, Anxa11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Annexin A11, Anxa11 ELISA KIT allows for the in vitro quantitative determination of Mouse Anxa11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Annexin A11, Anxa11 ELISA KIT has been pre-coated with an Mouse Annexin A11, Anxa11 antibody specific to Mouse Anxa11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Anxa11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Anxa11 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Anxa11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
酶的活性。Intergen公司的TRAP-eze Telemerase Detection Kit在1996年率先推出。它提供特定的寡核苷酸底物，分别与底物及端粒重复序列配对的引物。如果待测样本中含有端粒酶活性，就能在底物上接上不同个数的6碱基(GGTTAG)端粒重复序列，通过PCR反应，产物电泳检测就可观察到相差六个碱基的DNA Ladder现象(参见图4)。此外，Intergen公司还提供用酶联免疫法(ELISA)检测的试剂盒. 同样，这种检测方法也不专对细胞凋亡，检测结果也不纯反应细胞凋亡的发生
重复序列，通过PCR反应，产物电泳检测就可观察到相差六个碱基的DNA Ladder现象。此外，Intergen公司还提供用酶联免疫法（ELISA）检测的试剂盒。同样，这种检测方法也不专对细胞凋亡，检测结果也不纯反应细胞凋亡的发生。4、mRNA水平的检测研究者们发现了很多在细胞凋亡时表达异常的基因，检测这些特异基因的表达水平也成为检测细胞凋亡的一种常用方法。据报道，Fas 蛋白结合受体后能诱导癌细胞中的细胞毒性T细胞（cytotoxic T cells）等靶细胞。Bcl-2 和bcl-X （长的） 作为抗凋亡
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