- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-42948m
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ras-related protein Ral-B, Ralb
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse RALB ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse RALB ELISA KIT ,Mouse RALB Ras like proto-oncogene B ELISA KIT ,Mouse ras-related protein Ral-B ELISA KIT ,Mouse RAS-like protein B ELISA KIT ,Mouse ras related GTP binding protein B ELISA KIT ,Mouse v-ral simian leukemia viral oncogene homolog B (ras related GTP binding protein) ELISA KIT ,Mouse RAS like proto-oncogene B ELISA KIT
Intended use
Mouse RALB ELISA kit allows for the in vitro quantitative determination of Mouse RALB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse RALB ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RALB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RALB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RALB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse RALB ELISA kit Range 0.15-10ng/ml
RALB Related Products
> RALB ELISA KITS
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Mouse Ras- related protein Ral- B, Ralb ELISA KIT
Rat Ras- related protein Ral- B, Ralb ELISA KIT
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: 3128136151
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
Alternate Protocol 1: RNA Preparation from Pure RPE Cells Basic Protocol 4: Protein Preparation from Eye Tissues Basic Protocol 5: Fixation, Inclusion, and Cryosection of the Mouse Eye
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