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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
SH3 domain-containing protein 21, Sh3d21
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT
Product Name:Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT
Packing:96T
Catalog No.:ELI-42348m
Gene Name:Mouse Sh3d21
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Sh3d21
Alternative Name:Mouse SH3 domain-containing protein 21, Sh3d21
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT allows for the in vitro quantitative determination of Mouse Sh3d21 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT has been pre-coated with an Mouse SH3 domain-containing protein 21, Sh3d21 antibody specific to Mouse Sh3d21 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sh3d21 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sh3d21 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sh3d21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries
. These protein-protein interactions are often mediated by conserved-modular domains, such as the Src Homology domains 2 and 3, known as SH2 and SH3 (1 ,2 ). Recently, combmatorral-peptrde libraries have been used to define high-affinity peptide ligands
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
can be effectively homogenized by pipetting up and down or vortexing after addition of Cell lysis buffer. 3. Place the tube containing the homogenates on the bench at room temperature for 2 minutes. 4. Add 100 μL of Protein Precipitation Reagent
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