SH3 domain-containing protein
21, Sh3d21 ELISA KIT/ Mouse SH3 domain-containing protein 21, Sh3d21 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-42348m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
SH3 domain-containing protein 21, Sh3d21
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT
Product Name:Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT
Packing:96T
Catalog No.:ELI-42348m
Gene Name:Mouse Sh3d21
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Sh3d21
Alternative Name:Mouse SH3 domain-containing protein 21, Sh3d21
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT allows for the in vitro quantitative determination of Mouse Sh3d21 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse SH3 domain- containing protein 21, Sh3d21 ELISA KIT has been pre-coated with an Mouse SH3 domain-containing protein 21, Sh3d21 antibody specific to Mouse Sh3d21 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sh3d21 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sh3d21 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sh3d21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries
. These protein-protein interactions are often mediated by conserved-modular domains, such as the Src Homology domains 2 and 3, known as SH2 and SH3 (1 ,2 ). Recently, combmatorral-peptrde libraries have been used to define high-affinity peptide ligands
SH结构域是“Src同源结构域”(Src homology domain)的缩写(Src是一种癌基因,最初在Rous sarcoma virus 中发现)。这种结构域是能够与受体酪氨酸激酶磷酸化残基紧紧结合,形成多蛋白的复合物进行信号转导。 SH2大约由100个氨基酸组成。SH2结构域能够与生长因子受体(如PDGF和EGF)自我磷酸化的位点结合。含有SH2结构域的蛋白也常常含有SH3结构域。SH3结构域最初也是在Src中鉴定到的由50个氨基酸组成的组件,后来
Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases
(3 ). Nonreceptor tyrosine kinases such as Src and Abl are also targeted to subsets of cellular proteins by virtue of specific interactions with their SH2 and SH3 domains. The Src SH3 domain recognizes a proline-rich motif in an actin filament-assoctated protein
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