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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Tyrosine-protein kinase Fyn, Fyn
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Tyrosine- protein kinase Fyn, Fyn ELISA KIT
Product Name:Mouse Tyrosine- protein kinase Fyn, Fyn ELISA KIT
Packing:96T
Catalog No.:ELI-37594m
Gene Name:Mouse Fyn
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Fyn
Alternative Name:Mouse Tyrosine-protein kinase Fyn, Fyn
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Tyrosine- protein kinase Fyn, Fyn ELISA KIT allows for the in vitro quantitative determination of Mouse Fyn concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Tyrosine- protein kinase Fyn, Fyn ELISA KIT has been pre-coated with an Mouse Tyrosine-protein kinase Fyn, Fyn antibody specific to Mouse Fyn .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fyn and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fyn , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fyn in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
:
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文献和实验Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry
with the CD4 and CD8 coreceptors. Indeed, T cells from mice deficient in the expression of this enzyme fail to display a biochemical response in response to antigen-receptor crosslinking (1 ,2 ). The second kinase identified is p59fyn , as demonstrated
Analysis of Mitogen-Activated Protein Kinase Activation
The biological functions of mast cells are regulated by several protein kinases, including the tyrosine kinases Fyn, Lyn, Syk, and FAK and the serine/threonine kinases Akt and PKC α/β. The mitogen-activated protein kinases extracellular
Visualization of Protein Kinase Activities in Living Cells
kinase activity for a specific period in a single cell. Genetically encoded biosensors based on the principle of F�rster (or fluorescence) resonance energy transfer (FRET) and fluorescent proteins have been developed to accommodate
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