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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
C-C motif chemokine 6, Ccl6
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse C- C motif chemokine 6, Ccl6 ELISA KIT
Product Name:Mouse C- C motif chemokine 6, Ccl6 ELISA KIT
Packing:96T
Catalog No.:ELI-50760m
Gene Name:Mouse Ccl6
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Ccl6
Alternative Name:Mouse C-C motif chemokine 6, Ccl6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse C- C motif chemokine 6, Ccl6 ELISA KIT allows for the in vitro quantitative determination of Mouse Ccl6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse C- C motif chemokine 6, Ccl6 ELISA KIT has been pre-coated with an Mouse C-C motif chemokine 6, Ccl6 antibody specific to Mouse Ccl6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ccl6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ccl6 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ccl6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
lavage. Vet. Clin. Small Anim. 33:69‐88. Blé, F.‐X, Cannet, C., Zurbruegg, S., Karmouty Quintana, H., Frossard, N., Trifilieff, A., and Beckmann, N. 2008. Allergen‐induced lung
two to three colonies from each agar plate and set upa small volume (typically 5 ml) LB culture for miniprep. Incubate and shake under 37 °C overnight, prepare plasmidDNA using a spin miniprep kit according to manufacturer’sprotocol. 6. (Optional) Digest
细胞, 嗜酸性细胞, 嗜碱细胞, 自然杀伤细胞, 树突状细胞 CCL6 SCYA6 C10 (mouse), MRP-1 (mouse) 巨噬细胞 CCL7 SCYA7 MCP-3 单核细胞, T细胞, 嗜酸性细胞, 嗜碱细胞, 自然杀伤细胞, 树突
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