B- and T-lymphocyte attenuator

Mouse B- and T- lymphocyte attenuator, Btla ELISA KIT

Product Name:Mouse B- and T- lymphocyte attenuator, Btla ELISA KIT
Packing:96T

Catalog No.:ELI-34627m

Gene Name:Mouse Btla

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Btla

Alternative Name:Mouse B-and T-lymphocyte attenuator, Btla
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse B- and T- lymphocyte attenuator, Btla ELISA KIT allows for the in vitro quantitative determination of Mouse Btla concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse B- and T- lymphocyte attenuator, Btla ELISA KIT has been pre-coated with an Mouse B-and T-lymphocyte attenuator, Btla antibody specific to Mouse Btla .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Btla and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Btla , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Btla in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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